Zebrafish (Danio rerio) is a favorite model for studying development, in part because its transparent embryos make it possible to produce an ever-growing array of amazingly informative images. For one recent example, check out this Federation of American Societies for Experimental Biology’s 2016 BioArt winner, which shows the developing face of a 6-day-old zebrafish larva.
Yes, those downturned “lips” are indeed cells that will go on to become the fish’s mouth. But all is not quite what it appears: the two dark circles that look like eyes are actually developing nostrils. Both the nostrils and mouth express high levels of F-actin (green), a structural protein that helps orchestrate cell movement. Meanwhile, the two bulging areas on either side of the fish’s head, which are destined to become eyes and skin, express keratin (red).
Oscar Ruiz, who works in the lab of George Eisenhoffer at The University of Texas MD Anderson Cancer Center, Houston, used a confocal microscope to create this image. What was most innovative about his work was not the microscope itself, but how he prepared the sample for imaging. With traditional methods, researchers can only image the faces of zebrafish larvae from the side or the bottom. However, the Eisenhoffer lab has devised a new method of preparing fish larvae that makes it possible to image their faces head-on. This has enabled the team to visualize facial development at much higher resolution than was previously possible.
Tags: art, birth defects, cancer, cleft lip, cleft lip and palate, cleft palate, confocal microscope, craniofacial development, Danio rerio, development, epithelial cancers, epithelium, F-actin, FASEB Bioart 2016, model organism, Science, zebrafish
If you’ve ever tried to take photos of wiggly kids, you know that it usually takes several attempts before you get the perfect shot. It’s often the same for biomedical researchers when taking images with microscopes because there are so many variables—from sample preparation to instrument calibration—to take into account. Still, there are always exceptions where everything comes together just right, and you are looking at one of them! On her first try at using a confocal microscope to image this cross-section of a mouse embryo’s torso, postdoc Shachi Bhatt captured a gem of an image that sheds new light on mammalian development.
Bhatt, who works in the NIH-supported lab of Paul Trainor at the Stowers Institute for Medical Research, Kansas City, MO, produced this micrograph as part of a quest to understand the striking parallels seen between the development of the nervous system and the vascular system in mammals. Fluorescent markers were used to label proteins uniquely expressed in each type of tissue: reddish-orange delineates developing nerve cells; gray highlights developing blood vessels; and yellow shows where the nerve cells and blood vessels overlap.
Tags: birth defects, blood vessels, cleft lip, cleft lip and palate, cleft palate, confocal microscope, craniofacial biology, craniofacial development, developmental biology, FASEB Bioart 2015, mammalian development, Med23, microscopy, mouse, nervous system, vasculature